The United States Agency for International Development (USAID) has announced it will close its offices in Russia following an order from the authorities there to cease operations.
The Russian government gave the US until 1 October to close the mission.
USAID has worked in Russia for two decades, spending nearly $3bn (£1.8bn) on aid and democratic programmes.
Correspondents say the government's antipathy towards pro-democracy groups may be to blame for the move.
The Russian authorities have become increasingly suspicious of non-governmental organisations (NGOs), which it believes are using foreign funding to foment political unrest, says the BBC's
Earlier this year, President Vladimir Putin alleged that protests surrounding his re-election were orchestrated by US-funded NGOs.
Among the groups likely to be affected is Golos, whose exposure of electoral fraud at last year's parliamentary elections helped spark huge anti-Kremlin street demonstrations.
In announcing the closure of the USAID office, US state department spokesperson Victoria Nuland said: "We remain committed to supporting democracy, human rights, and the development of a more robust civil society in Russia and look forward to continuing our cooperation with Russian non-governmental organisations."
She would not be drawn on the reasons behind the Kremlin's decision, but said there was a sense "that they don't need this any more".
The United States began its operations in Russia after the end of the Soviet Union, spending around $2.7bn (£1.7bn) on a wide range of human rights, civil society, health and environmental programmes.
USAID was due to spend around $50m on its work in Russia this year.
BBC
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